[6]-Gingerol Suppresses Colon Cancer Growth by Targeting Leukotriene A4 Hydrolase

Abstract
[6]-Gingerol, a natural component of ginger, exhibits antiinflammatory
and antitumorigenic activities. Despite its
potential efficacy in cancer, the mechanism by which [6]-
gingerol exerts its chemopreventive effects remains elusive.
The leukotriene A4 hydrolase (LTA4H) protein is regarded as a
relevant target for cancer therapy. Our in silico prediction
using a reverse-docking approach revealed that LTA4H
might be a potential target of [6]-gingerol. We supported
our prediction by showing that [6]-gingerol suppresses
anchorage-independent cancer cell growth by inhibiting
LTA4H activity in HCT116 colorectal cancer cells. We showed
that [6]-gingerol effectively suppressed tumor growth in vivo
in nude mice, an effect that was mediated by inhibition of
LTA4H activity. Collectively, these findings indicate a crucial
role of LTA4H in cancer and also support the anticancer
efficacy of [6]-gingerol targeting of LTA4H for the prevention
of colorectal cancer. [Cancer Res 2009;69(13):5584–91]